Long-Term Alterations in Pulmonary V˙O2 and Muscle Deoxygenation On-Kinetics During Heavy-Intensity Exercise in Competitive Youth Cyclists: A Cohort Study.

PURPOSE: The aim of this investigation was to assess alterations of pulmonary oxygen uptake (V˙O2) and muscle deoxygenation on-kinetics during heavy-intensity cycling in youth cyclists over a period of 15 months. METHODS: Eleven cyclists (initial age, 14.3 [1.6] y; peak V˙O2, 62.2 [4.5] mL·min-1·kg-1) visited the laboratory twice on 3 occasions within 15 months. Participants performed an incremental ramp exercise test and a constant workrate test within the heavy-intensity domain during the first visit and second visit, respectively. Subsequently, parameter estimates of the V˙O2 and muscle deoxygenation on-kinetics were determined with mono-exponential models. RESULTS: The V˙O2 phase II time constant decreased from occasion 1 (34 [4] s) to occasion 2 (30 [4] s, P = .005) and 3 (28 [4] s, P = .010). However, no significant alteration was observed between occasions 2 and 3 (P = .565). The V˙O2 slow component amplitude either expressed in absolute values (ie, L·min-1) or relative to end exercise V˙O2 (ie, %) showed no significant changes throughout the study (P = .972 and .996). Furthermore, the muscle deoxygenation on-kinetic mean response time showed no significant changes throughout the study (18 [8], 18 [3], and 16 [5] s for occasions 1, 2, and 3, respectively; P = .279). CONCLUSION: These results indicate proportional enhancements of local muscle oxygen distribution and utilization, which both contributed to the speeding of the V˙O2 on-kinetics herein.

Alterations in aerobic fitness and muscle deoxygenation during ramp incremental exercise in trained youth cyclists: a ~3-year longitudinal study.

Alterations of aerobic fitness and muscle deoxygenation during a ramp incremental exercise test (GXT) were assessed on two occasions within a time-frame of 2.9 ± 0.1y in competitive youth cyclists. Nine cyclists (age, 14.5 ± 1.1y; peak oxygen uptake (V˙O2peak), 62.6 ± 4.2 mL.min-1.kg-1) participated in this investigation. V˙O2peak, the gas exchange threshold (GET) and the respiratory compensation point (RCP), as well as the muscle deoxygenation response pattern were determined during a GXT using open circuit spirometry and near-infrared spectroscopy, respectively. T-tests and Pearson's correlations were used to assess effects of time on the dependent variables and relationships between changes of parameter estimates of aerobic fitness and the muscle deoxygenation response, respectively. Workrate and metabolic rate at GET (33 ± 20 and 42 ± 23%) and RCP (36 ± 20 and 40 ± 22%), and V˙O2peak (30 ± 18%) significantly increased throughout the study (P < 0.05). The muscle deoxygenation response showed a significant rightward shift from occasion one to two (P < 0.05). Alterations in the workrate/metabolic rate at RCP, and V˙O2peak, were correlated with alterations of the muscle deoxygenation response (R = 0.71-0.89, P < 0.05). Together, this is thought to indicate a superior muscle perfusion within the tissue of interrogation at the same metabolic rate on occasion two vs. one, which partially contributed to the improved aerobic fitness in the cyclists herein.

Longitudinal alterations of pulmonary V . O2 on-kinetics during moderate-intensity exercise in competitive youth cyclists are related to alterations in the balance between microvascular O2 distribution and muscular O2 utilization.

Purpose: The main purpose of the current study was to investigate the dynamic adjustment of pulmonary oxygen uptake ( V . O2) in response to moderate-intensity cycling on three occasions within 15 months in competitive youth cyclists. Furthermore, the muscle Δdeoxy[heme] on-kinetics and the Δdeoxy[heme]-to- V . O2 ratio were modeled to examine possible mechanistic basis regulating pulmonary V . O2 on-kinetics. Methods: Eleven cyclists (initial age, 14.3 ± 1.6 y; peak V . O2, 62.2 ± 4.5 mL.min-1.kg-1) with a training history of 2-5 years and a training volume of ~10 h per week participated in this investigation. V . O2 and Δdeoxy[heme] responses during workrate-transitions to moderate-intensity cycling were measured with breath-by-breath spirometry and near-infrared spectroscopy, respectively, and subsequently modeled with mono-exponential models to derive parameter estimates. Additionally, a normalized Δdeoxy[heme]-to- V . O2 ratio was calculated for each participant. One-way repeated-measures ANOVA was used to assess effects of time on the dependent variables of the responses. Results: The V . O2 time constant remained unchanged between the first (~24 s) and second visit (~22 s, P > 0.05), whereas it was significantly improved through the third visit (~13 s, P = 0.006-0.013). No significant effects of time were revealed for the parameter estimates of the Δdeoxy[heme] response (P > 0.05). A significant Δdeoxy[heme]-to- V . O2 ratio "overshoot" was evident on the first (1.09 ± 0.10, P = 0.006) and second (1.05 ± 0.09, P = 0.047), though not the third (0.97 ± 0.10, P > 0.05), occasion. These "overshoots" showed strong positive relationships with the V . O2 time constant during the first (r = 0.66, P = 0.028) and second visit (r = 0.76, P = 0.007). Further, strong positive relationships have been observed between the individual changes of the fundamental phase τp and the Δdeoxy[heme]-to- V . O2 ratio "overshoot" from occasion one to two (r = 0.70, P = 0.017), and two to three (r = 0.74, P = 0.009). Conclusion: This suggests that improvements in muscle oxygen provision and utilization capacity both occurred, and each may have contributed to enhancing the dynamic adjustment of the oxidative "machinery" in competitive youth cyclists. Furthermore, it indicates a strong link between an oxygen maldistribution within the tissue of interrogation and the V . O2 time constant.

Effects of Flat and Uphill Cycling on the Power-duration Relationship.

The purpose of this study was to investigate the effects of flat and uphill cycling on critical power and the work available above critical power. Thirteen well-trained endurance athletes performed three prediction trials of 10-, 4- and 1-min in both flat (0.6%) and uphill (9.8%) cycling conditions on two separate days. Critical power and the work available above critical power were estimated using various mathematical models. The best individual fit was used for further statistical analyses. Paired t-tests and Bland-Altman plots with 95% limits of agreement were applied to compare power output and parameter estimates between cycling conditions. Power output during the 10- and 4-min prediction trial and power output at critical power were not significantly affected by test conditions (all at p>0.05), but the limits of agreement between flat and uphill cycling power output and critical power estimates are too large to consider both conditions as equivalent. However, power output during the 1-min prediction trial and the work available above critical power were significantly higher during uphill compared to flat cycling (p<0.05). The results of this investigation indicate that gradient affects cycling time-trial performance, power output at critical power, and the amount of work available above critical power.

Oxygen Uptake Kinetics in Endurance Trained Youth and Adult Cyclists.

Previous studies reported faster pulmonary oxygen uptake kinetics at the onset of exercise in untrained youth compared with adults. Whether or not these differences are identical for trained groups have not been examined. The purpose of this study was to compare ˙VO2 kinetics of youth and adult cyclists at moderate and heavy-intensity exercise. Thirteen adult (age: 23.2 ± 4.8 years; ˙VO2peak 68.4 ± 6.8 mL·min-1.kg-1) and thirteen youth cyclists (age: 14.3 ± 1.5 years; ˙VO2peak 61.7 ± 4.3 mL·min-1.kg-1) completed a series of 6-min square wave exercises at moderate and heavy-intensity exercise at 90 rev·min-1. A two-way repeated-measure ANOVA was conducted to identify differences between groups and intensities. The time constant, time delay and the mean response time were not significantly different between youth and adult cyclists (p > 0.05). We found significant differences between intensities, with a faster time constant during moderate than heavy-intensity exercise in youth (24.1 ± 7.0 s vs. 31.8 ± 5.6 s; p = 0.004) and adults (22.7 ± 5.6 s vs. 28.6 ± 5.7 s; p < 0.001). The present data suggest that the effect of training history in adult cyclists compensate for the superior primary response of the oxygen uptake kinetics typically seen in youth compared to adults. Furthermore, the ˙VO2 response is dependent of work rate intensity in trained youth and adult cyclists.

Aerobic and Anaerobic Power Distribution During Cross-Country Mountain Bike Racing.

PURPOSE: To determine aerobic and anaerobic demands of mountain bike cross-country racing. METHODS: Twelve elite cyclists (7 males; V˙O2max = 73.8 [2.6] mL·min-1·kg-1, maximal aerobic power [MAP] = 370 [26] W, 5.7 [0.4] W·kg-1, and 5 females; V˙O2max = 67.3 [2.9] mL·min-1·kg-1, MAP = 261 [17] W, 5.0 [0.1] W·kg-1) participated over 4 seasons at several (119) international and national races and performed laboratory tests regularly to assess their aerobic and anaerobic performance. Power output, heart rate, and cadence were recorded throughout the races. RESULTS: The mean race time was 79 (12) minutes performed at a mean power output of 3.8 (0.4) W·kg-1; 70% (7%) MAP (3.9 [0.4] W·kg-1 and 3.6 [0.4] W·kg-1 for males and females, respectively) with a cadence of 64 (5) rev·min-1 (including nonpedaling periods). Time spent in intensity zones 1 to 4 (below MAP) were 28% (4%), 18% (8%), 12% (2%), and 13% (3%), respectively; 30% (9%) was spent in zone 5 (above MAP). The number of efforts above MAP was 334 (84), which had a mean duration of 4.3 (1.1) seconds, separated by 10.9 (3) seconds with a mean power output of 7.3 (0.6) W·kg-1 (135% [9%] MAP). CONCLUSIONS: These findings highlight the importance of the anaerobic energy system and the interaction between anaerobic and aerobic energy systems. Therefore, the ability to perform numerous efforts above MAP and a high aerobic capacity are essential to be competitive in mountain bike cross-country.

Test-retest reliability of pulmonary oxygen uptake and muscle deoxygenation during moderate- and heavy-intensity cycling in youth elite-cyclists.

To establish the test-retest reliability of pulmonary oxygen uptake (V̇O2), muscle deoxygenation (deoxy[haem]) and tissue oxygen saturation (StO2) kinetics in youth elite-cyclists. From baseline pedalling, 15 youth cyclists completed 6-min step transitions to a moderate- and heavy-intensity work rate separated by 8 min of baseline cycling. The protocol was repeated after 1 h of passive rest. V̇O2 was measured breath-by-breath alongside deoxy[haem] and StO2 of the vastus lateralis by near-infrared spectroscopy. Reliability was assessed using 95% limits of agreement (LoA), the typical error (TE) and the intraclass correlation coefficient (ICC). During moderate- and heavy-intensity step cycling, TEs for the amplitude, time delay and time constant ranged between 3.5-21.9% and 3.9-12.1% for V̇O2 and between 6.6-13.7% and 3.5-10.4% for deoxy[haem], respectively. The 95% confidence interval for estimating the kinetic parameters significantly improved for ensemble-averaged transitions of V̇O2 (p < 0.01) but not for deoxy[haem]. For StO2, the TEs for the baseline, end-exercise and the rate of deoxygenation were 1.0-42.5% and 1.1-5.5% during moderate- and heavy-intensity exercise, respectively. The ICC ranged from 0.81 to 0.99 for all measures. Test-retest reliability data provide limits within which changes in V̇O2, deoxy[haem] and StO2 kinetics may be interpreted with confidence in youth athletes.

Field-Derived Power-Duration Variables to Predict Cycling Time-Trial Performance.

PURPOSE: To evaluate the predictive validity of critical power (CP) and the work above CP (W') on cycling performance (mean power during a 20-min time trial; TT20). METHODS: On 3 separate days, 10 male cyclists completed a TT20 and 3 CP and W' prediction trials of 1, 4, and 10 min and 2, 7, and 12 min in field conditions. CP and W' were modeled across combinations of these prediction trials with the hyperbolic, linear work/time, and linear power inverse-time (INV) models. The agreement and the uncertainty between the predicted and actual TT20 were assessed with 95% limits of agreement and a probabilistic approach, respectively. RESULTS: Differences between the predicted and actual TT20 were "trivial" for most of the models if the 1-min trial was not included. Including the 1-min trial in the INV and linear work/time models "possibly" to "very likely" overestimated TT20. The INV model provided the smallest total error (ie, best individual fit; 6%) for all cyclists (305 [33] W; 19.6 [3.6] kJ). TT20 predicted from the best individual fit-derived CP, and W' was strongly correlated with actual TT20 (317 [33] W; r = .975; P < .001). The bias and 95% limits of agreement were 4 (7) W (-11 to 19 W). CONCLUSIONS: Field-derived CP and W' accurately predicted cycling performance in the field. The INV model was most accurate to predict TT20 (1.3% [2.4%]). Adding a 1-min-prediction trial resulted in large total errors, so it should not be included in the models.

Comparison of Oxygen Uptake Kinetics During Severe-intensity Laboratory and Field Cycling.

The O2-kinetic response to constant work rate exercise provides an insight into the adjustment of systemic oxygen transport and muscle metabolism. Whether O2-kinetics measured in laboratory conditions reflect O2-kinetics in field conditions has not yet been analysed. The aim of this study was to compare O2-kinetics between field and laboratory conditions. Thirteen competitive male cyclists (mean±SD age 23.3±4.1 years; V̇O2peak 68.2±4.7 mL.min-1.kg-1) completed two 6-min severe-intensity trials at 60 and 90 rev.min-1 in both conditions. Power output was measured with an SRM power meter and V̇O2 was measured with a portable gas analyser. The time constant (τ), the time delay (TD) and the amplitude (Amp) were resolved by least square regression, and the V̇O2 slow component (SC) was calculated as the difference between the end-exercise V̇O2 and Amp. To determine differences between the trials, a repeated-measure ANOVA was conducted. The Amp and end-exercise V̇O2 were significantly higher during field cycling whereas the SC were significantly higher during laboratory cycling (all at p<0.001). No significant differences were found for τ (p=0.24). Laboratory measures tend to underestimate the oxygen demand in field cycling. A higher cadence leads to greater oxygen demand in laboratory and field cycling.

Reliability of the parameters of the power-duration relationship using maximal effort time-trials under laboratory conditions.

The purpose of this study was to assess the reliability of critical power (CP) and the total amount of work accomplished above CP (W´) across repeated tests using ecologically valid maximal effort time-trials (TT) under laboratory conditions. After an initial incremental exercise test, ten well-trained male triathletes (age: 28.5 ± 4.7 years; body mass: 73.3 ± 7.9 kg; height: 1.80 ± 0.07 m; maximal aerobic power [MAP]: 329 ± 41 W) performed three testing sessions (Familiarization, Test I and Test II) each comprising three TT (12, 7, and 3 min with a passive recovery of 60 min between trials). CP and W´ were determined using a linear regression of power vs. the inverse of time (1/t) (P = W´ ∙ 1/t + CP). A repeated-measures ANOVA was used to detect differences in CP and W´ and reliability was assessed using the intra-class correlation coefficient (ICC) and the coefficient of variation (CoV). CP and W´ values were not significantly different between repeated tests (P = 0.171 and P = 0.078 for CP and W´, respectively). The ICC between Familiarization and Test I was r = 0.86 (CP) and r = 0.58 (W´) and between Tests I and II it was r = 0.94 (CP) and r = 0.95 (W´). The CoV notably decreased from 4.1% to 2.6% and from 25.3% to 8.2% for CP and W´, respectively. Despite the non-significant differences for both parameter estimates between Familiarization, Test I, and Test II, ICC and CoV values improved notably after the familiarization trial. Our novel findings indicate that for both, CP and W´ a familiarization trial increased reliability. It is therefore advisable to familiarize well-trained athletes when determining the power-duration relationship using TT under laboratory conditions.

Validity and Reliability of the Garmin Vector Power Meter in Laboratory and Field Cycling.

To assess the validity and reliability of the Garmin Vector against the SRM power meter, 6 cyclists completed 3 continuous trials at power outputs from 100-300 W at 50-90 rev·min-1 and a 5-min time trial in laboratory and field conditions. In field conditions only, a 30-s sprint was performed. Data were compared with paired samples t-tests, with the 95% limits of agreement (LoA) and the typical error. Reliability was calculated as the coefficient of variation (CV). There was no significant difference between the devices in power output in laboratory (p=0.245) and field conditions (p=0.312). 1-s peak power was significantly different between the devices (p=0.043). The LoA were ~1.0±5.0 W and ~0.5±0.5 rev·min-1 in both conditions. The LoA during the 30-s sprint was 6.3±38.9 W and for 1-s peak power it was 18.8±17.1 W. The typical error for power output was 2.9%, while during sprint cycling it was 7.4% for 30-s and 2.7% for 1-s peak power. For cadence, the typical error was below 1.0%. The mean CVs were ~1.0% and ~3.0% for the SRM and Garmin, respectively. These findings suggest, that the Garmin Vector is a valid alternative for training. However, during sprint cycling there is lower agreement with the SRM power meter. Both devices provide good reliability (CV<3.0%).

Validity of Treadmill-Derived Critical Speed on Predicting 5000-Meter Track-Running Performance.

Nimmerichter, A, Novak, N, Triska, C, Prinz, B, and Breese, BC. Validity of treadmill-derived critical speed on predicting 5,000-meter track-running performance. J Strength Cond Res 31(3): 706-714, 2017-To evaluate 3 models of critical speed (CS) for the prediction of 5,000-m running performance, 16 trained athletes completed an incremental test on a treadmill to determine maximal aerobic speed (MAS) and 3 randomly ordered runs to exhaustion at the [INCREMENT]70% intensity, at 110% and 98% of MAS. Critical speed and the distance covered above CS (D') were calculated using the hyperbolic speed-time (HYP), the linear distance-time (LIN), and the linear speed inverse-time model (INV). Five thousand meter performance was determined on a 400-m running track. Individual predictions of 5,000-m running time (t = [5,000-D']/CS) and speed (s = D'/t + CS) were calculated across the 3 models in addition to multiple regression analyses. Prediction accuracy was assessed with the standard error of estimate (SEE) from linear regression analysis and the mean difference expressed in units of measurement and coefficient of variation (%). Five thousand meter running performance (speed: 4.29 ± 0.39 m·s; time: 1,176 ± 117 seconds) was significantly better than the predictions from all 3 models (p < 0.0001). The mean difference was 65-105 seconds (5.7-9.4%) for time and -0.22 to -0.34 m·s (-5.0 to -7.5%) for speed. Predictions from multiple regression analyses with CS and D' as predictor variables were not significantly different from actual running performance (-1.0 to 1.1%). The SEE across all models and predictions was approximately 65 seconds or 0.20 m·s and is therefore considered as moderate. The results of this study have shown the importance of aerobic and anaerobic energy system contribution to predict 5,000-m running performance. Using estimates of CS and D' is valuable for predicting performance over race distances of 5,000 m.

Gross efficiency during flat and uphill cycling in field conditions.

PURPOSE: While a number of studies have investigated gross efficiency (GE) in laboratory conditions, few studies have analyzed it in field conditions. Therefore, the aim of this study was to analyze the effect of gradient and cadence on GE in field conditions. METHODS: Thirteen trained cyclists (mean ± SD age 23.3 ± 4.1 y, stature 177.0 ± 5.5 cm, body mass 69.0 ± 7.2 kg, maximal oxygen uptake [VO2max] 68.4 ± 5.1 mL · min-1 ·kg-1) completed an incremental graded exercise test to determine ventilatory threshold (VT) and 4 field trials of 6 min duration at 90% of VT on flat (1.1%) and uphill terrain (5.1%) with 2 different cadences (60 and 90 rpm). VO2 was measured with a portable gas analyzer and power output was controlled with a mobile power crank that was mounted on a 26-in mountain bike. RESULTS: GE was significantly affected by cadence (20.6% ± 1.7% vs 18.1% ± 1.3% at 60 and 90 rpm, respectively; P < .001) and terrain (20.0% ± 1.5% vs 18.7% ± 1.7% at flat and uphill cycling, respectively; P = .029). The end-exercise VO2 was 2536 ± 352 and 2594 ± 329 mL/min for flat and uphill cycling, respectively (P = .489). There was a significant difference in end-exercise VO2 between 60 (2352 ± 193 mL/min) and 90 rpm (2778 ± 431 mL/min) (P < .001). CONCLUSION: These findings support previous laboratory-based studies demonstrating reductions in GE with increasing cadence and gradient that might be attributed to changes in muscle-activity pattern.